Isolation and expression of a cold-responsive gene PtCBF in Poncirus trifoliata and isolation of citrus CBF promoters
C-repeat/dehydration-responsive element binding factor (CBF) plays important roles in cold response network in plants.Here, one member of CBF coding gene family in trifoliate orange (Poncirus trifoliata), designated as PtCBF, was isolated.Semi-quantitative reverse transcription-polymerase chain reactions showed up-regulation of PtCBF not only under